Jan 23 2008 Lab Exercise 2 After Practicing Lab

jan 23 2008 lab exercise 2 after practicing lab exercise 1, you have learned a gene called pen-2 existing in d. melanogaster, c. ele

Jan 23 2008
Lab exercise 2
After practicing lab exercise 1, you have learned a gene called pen-2
existing in D. melanogaster, C. elegans, mouse and human. In this
exercise, you are going to do the following:
1.
List amino acid sequences of Pen-2 protein in D. melanogaster, C.
elegans, mouse and human.
2.
List cDNA sequences of pen-2 in D. melanogaster, C. elegans, mouse
and human. Explain how you find these sequences.
3.
List genomic DNA sequences of pen-2 in D. melanogaster, C. elegans,
mouse and human, each with clear indication of exon/intron
structure, and positions of start codon and stop codon. Do these
four species have same number of exons? Explain how you find these
sequences.
4.
Compose the homology comparison of the Pen-2 proteins of D.
melanogaster, C. elegans, mouse and human, and Identify the
conserved region(s) among these orthologs. Overall, what is the
percentage of homology among these four? How about between D.
melanogaster, and human, and between mouse and human?
5.
Compose the homology comparison of the pen-2 cDNAs of D.
melanogaster, C. elegans, mouse and human, and Identify the
conserved region(s), if any, among these orthologs.
6.
In the case of pen-2 gene, does the conservation among all
orthologs mentioned above exist in the protein level or DNA level?
What about for other genes?
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